Abstract: Ninety- six samples of newly reclaimed soil were collected from around the main 8 lakes of Wadi El-Natrun region. Chemical analysis of these samples revealed that they are alkaline with pH 8.2–9.05, organic matter content from 0.3 to 2.0% and total soluble salts from 1.7 - 6.3%. Soil collected from Al Gaar Lake showed the highest levels of total soluble salts, potassium, sodium, calcium, magnesium carbonate, bicarbonate, and chlorides. On the other hand, some parameters showed their peaks in other lakes, such as sodium (11.1 mg/g) in Umm Risha, calcium (0.2 mg/g) in Hamra and magnesium (0.04 mg/g) in AL Beida. A total number of genera (31) and species and varieties (84+3) were recorded from soil samples collected from all lakes during the study on the three isolation media used, with the widest spectrum of species being isolated on Czapek´s medium (60+2) and the narrowest on 10% NaCl medium (41+1), whereas 40% sucrose medium was intermediate (49+2). Aspergillus (17+2 species varieties), Fusarium (14), Myrothecium (3), Stachybotrys (2), Penicillium (8) and Emericella (4) were regularly the most dominant genera possessing the highest proportions of propagules on the three isolation media. Aspergillus niveus, Chaetomium globosum, C. olivaceum, C. monocereus, Fusarium poae, Scopulariopsis (4 species), Scytalidium lignicola and Thermoascus aurantiacus were isolated only on 10% NaCl medium. Aspergillus showed its count peak in Khadra Lake on the control medium, in Fasida on 40% sucrose and 10% NaCl medium. A. flavus, A. fumigatus, A. niger, A. ochraceus, A. sydowii and A. terreus, in addition to P. chrysogenum and F. solani were common on all isolation media. Emericella and Eurotium were the most common genera on 40% sucrose. The data of the present study showed that the majority of fungal species recovered from soil around the 8 lakes of Wadi El-Natrun are osmotolerant/osmophilic and halotolerant/halophilic. Key words: Alkaline soil around 8 lakes, Wadi El-Natrun, osmotolerant/osmophilic and halotolerant/halophilic fungi.