Abstract: In the present study, 145 women were clinically examined during the period from December 2011 to July 2012 for vaginal yeast infection. Direct microscopy and culturing of vaginal swabs revealed that only 93 cases (64.1 %) were confirmed to be affected by yeasts. The majority of patients were 21-40 years old representing 70% of the positive cases. Yeast infection was more encountered in women receiving oral contraceptives (40%) than in those complaining of diabetes mellitus (25%) or treated with corticosteroids (17%). Phenotypic and genotypic characterization of yeast isolates showed that Candida albicans was the most prevalent species affecting 45.2% of patients, followed by C. krusei and C. tropicalis (20.4 % and 10.8% respectively). C. glabrata and C. parapsilosis were rare (3.3% and 1.1% respectively). Rhodotorula mucilaginosa and Geotrichum candidum occurred in 18.3% and 1.1% of vaginal samples respectively. Protease was produced by 83 out of 93 isolates tested (89.2%) with active isolates belonging to C. albicans and C. krusei. Lipase was produced by 51.6% of isolates with active producers related to C. albicans, C. tropicalis, C. glabrata and C. krusei. All isolates of Candida and Geotrichum were unable to produce urease enzyme, but those of R. mucilaginosa produced this enzyme. In-vitro sensitivity test showed that C. albicans isolates were sensitive to all antifungal agents (12 types) with the most effective drugs being Nystatin and Terbinafine affecting 100% of strains. Cetrimide, Fluconazole, Clotrimazole, Miconazole and Amphotericin-B affected 59.5.8% - 85.7% of the tested isolates. Key words: vaginal candidiasis, antifungal agents, enzymes.