Abstract: A total of 200 specimens from infected eyes were collected and studied microbiologicaly during April 2005 to January 2006 from patients admitted in different hospitals in Taїz City. Eye infections were more frequent during April, August, September and December. 139 cases (69.5%) were infected by bacteria (126 cases) or fungi (7) or a mixture of bacteria and fungi (6). Eye infections in relation to sex revealed that female cases were higher than those of males. Infection in relation to age group indicated that those of age between 21-30 years were the most susceptible. Also, infections in relation to occupation of patients revealed that, the farmers and those who have no definite work were highly susceptible. A total of 7 fungal species related to 4 genera were identified of which Aspegillus species were the most prevalent. Ten species and 4 unidentified species of bacteria appertaining to 10 genera were recovered. Species of Staphylococcus, Pseudomonas aeruginosa and Escherichia coli were the most common agents of eye infections. Five species of fungi and 4 species and 1 unidentified species of bacteria were isolated from the mixed cases. Key words: Keratitis, fungi, bacteria, eye diseases, microorganisms