Abstract: Aspergillus assiutensis, a new species isolated from the air of a grapevine plantation at Sahel-Saleem, Assiut, is described and illustrated. It is characterized by fast-growing whitish colonies, uniseriate conidiogenous cells, globose to radiate heads, thick-walled stipes and vesicles, pyriform to elongate conidia when young and globose to subglobose at maturity. ITS sequence of the isolate was determined and strongly supported the morphological differences. A new section (section Assiuti) of subgenus Circumdati is proposed for the species since it does not fit typically with any of the known sections of that subgenus. Key words: Aspergillus assiutensis, Assiut, Egypt, air, grapevine plantation, ITS.