Abstract: Monthly samples were collected from water and submerged mud of As-Sant Canal 5 km North East of Assuit City, Egypt during the period from November 2012 to October 2013. Using sesame seedsas baits, 18 species belonging to 7 genera of zoosporic fungi were isolated from water. The broadest spectrum of these fungi (7 species) was observed in January whereas the narrowest (3 species) was in May. Achlya followed by Saprolegnia and Dictyuchus were the most common genera being recovered through 11, 9, and 7 months respectively. The genus Achlya prevailed during August to October 2013 due to the high counts of A. klebsiana. Saprolengia was dominant during November 2012 to May 2013 being affected by the counts of S. ferax and S. furcata. Dictyuchus monosporuswas appeared in relatively high counts during June. Other fungi including Aphanomyces, Pythium, Phytophthora and Thraustotheca were less frequently observed. Terrestrial mycobiotawere represented by 112 species and 49 generain case of water samples and 99 species belonging to 45 genera in case of submerged mud. Acremonium, Aspergillus, Fusarium, Galactomyces, Mucor, Pinicillium, Talaromyces and Trichoderma were the most common genera in water and mud samples. Keywords: Zoosporic and terrestrial fungi, fresh water and submerged mud.