Abstract: Aspergillus calidoustus is a human pathogen, causing an invasive infection to the immunocompromised patients. The occurrence of this fungus in the air had a serious effect on human health.A strain of Aspergillus related to section Usti was isolated from air at Assiut area, Egypt in 2003. The strain was grown on different media for morphological description, as well as, molecularly identified based on their ITS sequence.From the morphological description and molecular analysis, this strain has been confirmed as A. calidoustus, the well-known human pathogen. The strain was able to grow well at 37°C. To the best of our knowledge, this isthe first record of this species in Egypt.The strain was deposited in the culture collection of Assiut University Moubasher Mycological Centre as AUMC 2007 (=CCF 5184 in culture collection of fungi at the Department of Botany, Prague)and the ITS gene sequence was deposited at the National Centre of Biotechnology Information (NCBI) with GenBank accession number: MK729534.A brief description of the fungus was presented. Key words: Human pathogen, Aspergillus calidoustus, Phenotypiccriteria, Genotypic characterization.