Abstract: The ongoing research resulted in the isolation and identification of 38 endophytic mycobiota species from 20 genera associated with four wild and four cultivated plants collected from El-Kharga, New Valley Governorate, Egypt. The most prominent genera were Aspergillus, Alternaria, Fusarium, Exserohilum, and Monascus, accounting for 36.24 %, 11.13 %, 10.95 %, 7.35 %, and 4.25 % of total fungi, respectively. Aspergillus was found in 100 % of wild plants and 75 % of cultivated plants, Alternaria (50 % and 25 %), Fusarium (25 % and 75 %), Exserohilum (50 % and 25 %), and Monascus (50 % and 25 %). Wild plants had a higher overall CFU count (260/200 segments), number of genera (14) and species (26) than cultivated plants (187 CFUs/200 segments, 10 genera and 16 species). A. terreus and A. flavus were the most common on wild plant species, while F. oxysporum and F. solani were the most common on cultivated plant species. Wild plant-isolated fungi showed increased growth ability in endoglucanase, exoglucanase, and pectinase enzymes with powerful strains A. flavus AY-72 from Prosopis farcta produced acumulative output of 242.81 IU/ml/min of pectinase, dark sterile mycelia AY-133 from Trifolium sativum producing 14.6 IU/ml/min of endoglucanase and F. solani AY-126 from Ammi majus producing 65.5 IU/ml/min of exoglucanase. The current results stated that wild plants have more efficient enzyme-generating strain sources than cultivated plants, with higher enzyme activity. Kew words: Endophytic fungi, pectinase, cellulase, submerged fermentation, wild, cultivated, plants.