This study aimed to use maize stalks for preparation of compost using Aspergillus brasiliensis and Penicillium brevicompactum. The prepared compost was applied to promot maize growth. Fungal analysis during compost preparation resulted in isolation of sixteen fungal species belonging to 5 genera from maize stalks (MS), farmyard manure (FYM) and different treatments at both 28˚C (15 species and 5 genera) and 45˚C (6 and 2). Aspergillus, Fusarium and Penicillium were the most common genera. A. flavus, A. niger, A. brasiliensis, A. fumigatus, A. quadrilineatus, A. sydowii, A. ustus and F. solani represented the most predominant species. Both organic matter (OM) and organic carbon (OC) were declined during composting to 26.41, 15.3 (T1), 30.02, 17.4 (T2), 27.5, 15.9 (T3) and 31.42, 18.2 (T4), respectively. On the other hand, total N reached the maximum percentage after 60 days meanwhile, C/N ratio decreased during composting from 26.6 to reach 11.7, 10.8, 12.2 and 12.6 for T1, T2, T3 and T4 respectively. It is worthy to mention that, the best prepared compost was that treated with mixed culture of A. brasiliensis + P. brevicompactum (T4). This compost was applied in soil with different doses to improve maize growth. From the results, there was a significant increase in all measured parameters in different treatments, especially in case of treatment with 100% compost + 100% mineral N. Furthermore, the treatment mentioned above produced the best compost enriched the soil with organic matter and the availability of nitrogen, phosphorus and potassium.