Abstract: Six high ethanol producer yeast strains (two strains of Kluyveromyces marixianus and four of Saccharomyces cerevisiae) were utilized to produce ethanol from treated and non-treated Egyptian sugar cane molasses with gravity (10, 15, 20, 30 & 33.3% sugar). The treated molasses was obtained by heating diluted molasses up to 90ºC and adjusting its pH to 4.5. All yeast strains used produced higher ethanol yield from non-treated molasses with 10% sugar than that obtained from the treated one with the same sugar concentration. On the other hand, treated molasses yielded better ethanol concentration than that gained from non-treated molasses with 15 – 25% sugar. Maximum ethanol production (125.89% g/l) was noticed with fermentation efficiency of 99.97% using S. cerevisiae EC1118 strain on 25% sugar treated molasses at 35ºC. The same strain gave low levels of ethanol when the sugar concentration of the treated molasses was either 30 or 33.3% at both fermentation temperatures used (35º and 40ºC). The kinetic parameters and productivity were calculated and discussed for all treatments. Key words: batch fermentation, ethanol, sugar cane molasses, yeast